2 mercaptoethanol (Bio-Rad)

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Bio-Rad 2 mercaptoethanol
2 Mercaptoethanol, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 12140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/laemmli+sample+buffer/4x+Laemmli+Sample+Buffer/pmc13148924-213-25-27
Average 99 stars, based on 12140 article reviews

2 mercaptoethanol - by Bioz Stars, 2026-09

99/100 stars

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Article Title: RMC-6272, a selective third-generation bi-steric mTORC1 inhibitor, improves cardiac function in pressure overload-induced cardiac hypertrophy
Article Snippet: Equivalent amounts of protein, were brought up to similar volume, mixed with Laemmli Sample Buffer (Bio-Rad; 161-0747) and 2-Mercapoethanol (cat# M6250, Sigma-Aldrich) and boiled at 95 °C for 5 min.

Article Title: Loss of SALL1 Promotes Hepatocellular Carcinoma Growth and Is Associated with Poor Clinical Outcome.
Article Snippet: Protein lysates were mixed with 4× Laemmli Sample Buffer (Bio-Rad Laboratories, Hercules, CA, USA; Cat. No. 1610747) and heated at 95 ◦C for 5 min.

Article Title: A bifidobacterial enzyme orchestrates ecology and function of infant gut bacterial community
Article Snippet: Protein samples were prepared by resuspending 10 μL of 2× Laemmli Sample Buffer (Bio-Rad, 1610737) with 10 μL of the protein sample, followed by denaturing at 95 □°C for 5 minutes (ThermoFisher Thermomixer, 5436).

Lysis:

Article Title: An optimized translating ribosome affinity purification protocol for low-abundance Drosophila tissues
Article Snippet: .. The abdomen of two (2 day-old) male flies per sample was excised and discarded and the remaining tissues (thoraces, heads and legs) were lysed in RIPA lysis buffer (50 mM pH 7.4 Tris, 1 M NaCl, 0.1 % SDS, 0.5 % sodium deoxycholate, 1 % NP-40; cOmplete mini EDTA-free protease inhibitors (Roche; 4693159001)) and diluted with Laemmli Sample Buffer (Bio-Rad; 1610747). ..

Centrifugation:

Article Title: Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency
Article Snippet: .. The lysate was clarified by centrifugation at 10,000× g for 20 min at 4 °C, and the clarified supernatant was combined with 4× Laemmli Sample Buffer (Bio-Rad; 1610747) and heated at 95 °C for 10 min. .. Protein extracts were subsequently separated by electrophoresis at 150 V for 45 min on a NuPAGE 3–8% Tris-Acetate gel (Thermo Fisher Scientific; EA0378BOX) in 1× NuPAGE Tris-Acetate SDS running buffer (Thermo Fisher Scientific; LA0041).

Article Title: Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency.
Article Snippet: .. The lysate was clarified by centrifugation at 10,000× g for 20min at 4 °C, and the clarified supernatant was combined with 4× Laemmli Sample Buffer (Bio-Rad; 1610747) and heated at 95 °C for 10min. .. Protein extracts were subsequently separated by electrophoresis at 150 V for 45min on a NuPAGE 3–8% Tris-Acetate gel (Thermo Fisher Scientific; EA0378BOX) in 1× NuPAGE Tris-Acetate SDS running buffer (Thermo Fisher Scientific; LA0041).

Staining:

Article Title: Phosphorothioate antisense oligonucleotide induced innate immune activation is attenuated by tryptophan oxidation products
Article Snippet: Proteins were clarified by centrifugation at 14 000 g x 15 min at 4°C, with concentration determined using a Pierce BCA protein Assay Kit (Thermo Fisher, 23227). .. Clarified protein samples were prepared with Laemmli Sample Buffer (BioRad, 1610747) and 2-Mercaptoethanol (BioRad, 1610710), and ∼15 ug of each sample was separated on a 4–20% Mini-Protean TGX Stain-free Precast Gel (BioRad, 4568094). .. Separated protein samples were transferred onto 0.2 μm polyvinylidene difluoride (PVDF) membranes (BioRad, 1704156) using a Trans-Blot® TurboTM Transfer System (BioRad, 1704150).

Bicinchoninic Acid Protein Assay:

Article Title: MYC-bound enhancer RNAs in cis regulate gene transcription and tumorigenesis
Article Snippet: In brief, cells were directly lysed for 30 min on ice in the lysis buffer [50 mM tris-HCl, (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1% Triton X-100], freshly added PMSF to a final concentration of 1 mM, and 1× protease inhibitor cocktail (Sigma-Aldrich, P8340). .. The total protein was quantified by a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) and boiled at 95°C for 10 min with an equal volume of 2× Laemmli Sample Buffer (Bio-Rad, 161-0737). .. About 30 μg of proteins was resolved by SDS–polyacrylamide gel electrophoresis under denaturing conditions and transferred to polyvinylidene difluoride membranes (Bio-Rad, 162-0177).

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